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46 819 Ft
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Product details:
- Publisher OUP Oxford
- Date of Publication 2 July 1998
- ISBN 9780199636464
- Binding Paperback
- No. of pages288 pages
- Size 235x157x16 mm
- Weight 495 g
- Language English
- Illustrations 9 halftones, numerous line figures 0
Categories
Short description:
Growth factors are extra-cellular molecules that interact with receptors on the cell surface to initiate a sequence of events culminating in transcription of DNA prior to cell division. Disturbances in the production of growth factors or the sensitivity of their receptors are important in tumour growth. Therefore, there is much interest in engineering both growth factors and their receptors to intervene in cancer. This book describes a variety of techniques for the
engineering, analysis, definition of function and detection of gene expression of growth factors and their receptors. A number of protocols for the study of growth factor - receptor interactions and the gene therapy applications of growth factors in, for example, wound healing are also
described.
Long description:
Growth Factors and Receptors: A Practical Approach provides comprehensive protocols for studies of growth factors and their interactions with receptors. It covers a wide range from simple analytical techniques to sophisticated in vivo applications including: RT-PCR and immunocytochemistry for detection of growth factors and receptors; production and purification of recombinant growth factors and receptors; labelling of growth factors for binding studies; in vivo
mutagenesis; the yeast two-hybrid assay of proteinprotein interactions; phage display of factors; application of factors to wound-healing processes using the gene gun; treatment of cancers with factor/toxin chimeras; and analysis of important factor domains using chimeric proteins. This book updates
and extends the current literature and describes important novel approaches to the study of growth factors and their receptors, including the use of RNA aptamers as receptor antagonists, and the development of receptor superantagonists. It will be of tremendous value to both researchers and teachers, and, through an appendix that lists a large number of growth factors and receptors, will serve as a handy reference text.
Table of Contents:
Engineered growth factors and receptors, and their applications
Engineering of novel neurotrophins
Designing new agonists.antagonists of growth factor receptors - the rational design of a super-antagonist of the IL-6 receptor
A Chimeric approach for studying receptor-binding domains in EGF-like molecules
Structure-activity relationships of chemokines
Generation and selection of RNA ligands that inhibit interaction of vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) to its receptor
Immunolocalisation and RT-PCR for detection and quantification of growth factor and gene expression
Defining growth factor function through tissue-specific expression of dominant-negative receptor mutants
Growth factor-toxin chimeras and their applications
Gene therapy applications of growth factors
Appendix 1
Appendix 2
Appendix 3
