
Endocrine Cell Culture
Series: Handbooks in Practical Animal Cell Biology;
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Product details:
- Publisher Cambridge University Press
- Date of Publication 28 August 1998
- ISBN 9780521595636
- Binding Paperback
- No. of pages168 pages
- Size 229x152x9 mm
- Weight 230 g
- Language English
- Illustrations 45 b/w illus. 4 tables 0
Categories
Short description:
This book offers clear, affordable lab protocols and descriptive accounts of all major endocrine cell cultures.
MoreLong description:
This handy reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally-intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures which have been devised to ensure the optimal survival and behaviour of each cell type under study. Particular emphasis has been placed on the provision of detailed protocols describing, step by step, the manipulations necessary to establish differentiated and responsive cultures. The chapters have been prepared by authors having direct practical experience of the cell type concerned, and the reader is therefore provided with first-hand accounts on the background to each procedure, the avoidance of potential problems and pitfalls, and the fundamental questions in endocrinology which may be addressed using each cell culture model.
'No one other book presents a collection of such contributions of the highest interest for all cellular and molecular biologists and we are sure that this booklet will meet a true great success, what we sincerely wish to it!' R. Wegmann, Cellular and Molecular Biology
Table of Contents:
List of contributors; Preface; Acknowledgements; Introduction Stephen Bidey; 1. Parathyroid cells Ronal R. Macgregor; 2. Ovarian granulosa Denis A. Magoffin; 3. Anterior pituitary cells Seon H. Shin and John V. Milligan; 4. Pancreatic &&&223;-cells Leonard Best; 5. Adrenocortical and adrenomedullary cells Matthias M. Weber, Christian Fottner and Dieter Engelhardt; 6. Leydig cells Frank Chuzel, Herv&&&233; Lejeune and Jos&&&233; Saez; 7. Thyroid follicular cells Margaret C. Eggo; 8. Hypothalamic cells Hilary E. Murray, Duncan McKenzie and Glenda E. Gillies; Index.
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