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Diabetic Nephropathy: Methods and Protocols

Diabetic Nephropathy

Methods and Protocols
 
Edition number: 1st ed. 2020
Publisher: Humana
Date of Publication:
Number of Volumes: 1 pieces, Book
 
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Product details:
ISBN13:9781493998432
ISBN10:1493998439
Binding:Paperback
No. of pages:342 pages
Size:254x178 mm
Weight:677 g
Language:English
Illustrations: 14 Illustrations, black & white; 95 Illustrations, color; 93 Tables, color
261
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Short description:

This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and to develop treatments for this condition.

Long description:
This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and to develop treatments for this condition.
Table of Contents:

Part I: Introduction



 



1. Diabetic Nephropathy: An Overview



            Manpreet K. Sagoo and Luigi Gnudi



 



Part II: Experimental Models to Study Diabetic Nephropathy



 



2. Modeling Podocyte Biology Using Drosophila Nephrocytes



            Paul S. Hartley and Richard J. Coward



 



3. A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros



            Maria Kolatsi-Joannou and Daniel Osborn



 



4. Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model



            Carolynn Cairns and Bryan Conway



 



5. Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy



            Elisavet Vasilopoulou



 



Part III: Techniques to Image the Diabetic Kidney



 



6. Histological Examination of the Diabetic Kidney



            Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, and Gianfranco Marchetti



 



7. Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy



            Kathryn E. White



 



8. Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy



            Daniyal J. Jafree, David A. Long, Peter J. Scambler, and Dale Moulding



 



Part IV: Assessing the Function and Pathophysiological Properties of the Diabetic Kidney



 



9. Transcutaneous Measurement of Glomerular Filtration Rate in Rodents



            Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, and Norbert Gretz



 



10. An In Vitro Method to Analyze Glucose Uptake in Podocytes



            Abigail C. Lay and Richard J. Coward



 



11. Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro



            Raina D. Ramnath and Simon C. Satchell



 



12. Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy



            Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, and Berend Isermann



 



13. Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease



            Karina Barreiro, Tobias B. Huber, and Harry Holthofer



 



14. Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy



            Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, and Paul E. Squires



 



Part V: Transcriptome, Metabolic, and Proteomic Profiling of the Diabetic Kidney



 



15. Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches



            Laura J. Smyth, Katie Kerr, Seamus Duffy, Jill Kilner, and Amy Jayne McKnight



 



16. Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data - Association Studies



            Marisa Canadas-Garre, Laura J. Smyth, Kerry Anderson, Katie Kerr, and Amy Jayne McKnight



 



17. Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients



            Lucy Jade Newbury, Alexa Wonnacott, Kate Simpson, Timothy Bowen, and Donald Fraser



 



18. Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy



            Justyna Siwy, Linda Ahonen, Pedro Magalh?es, Maria Franzi, and Peter Rossing



 



Part VI: New Approaches: Gene Editing and Stem Cells to Study Diabetic Kidney Disease



 



19. Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice



            Ioannis Bantounas, Edina Silajdžić, Adrian S. Woolf, and Susan J. Kimber



 



20. Generating Mutant Renal Cell Lines Using CRISPR Technologies



            Nuria Perretta Tejedor, Grace M. Freke, Marian Seda, David A. Long, and Dagan Jenkins