Crystallization of Nucleic Acids and Proteins: A Practical Approach
Series: Practical Approach Series; 86;
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46 819 Ft
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Product details:
- Publisher IRL Press
- Date of Publication 27 February 1992
- ISBN 9780199632466
- Binding Paperback
- No. of pages360 pages
- Size 234x155x20 mm
- Weight 548 g
- Language English
- Illustrations halftones, line illustrations, tables 0
Categories
Short description:
In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules.
Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.
Long description:
In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules.
Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.
'an invaluable contribution to the literature in this research area ... it will be most useful for students and beginners in the field of crystallization ... this very up-to-date, comprehensive text ... is written and presented with great clarity. I can strongly recommend it as a standard text on crystallization that no laboratory involved in the field should be without.'
Lesley F. Lloyd, Imperial College of Science, Technology and Medicine, Tibtech, April 1993 (Vol. 11)
Table of Contents:
R. Giegé & A. Ducruix: An introduction to the crystallogenesis of biological macromolecules; B. Lorber & R. Giegé: Preparation and handling of biological macromolecules for crystallization; C.W. Carter: Design of crystallization experiments and protocols; A. Ducruix & R. Giegé: Methods of crystallization; E. Stura & I.A. Wilson: Seeding techniques; M.C. Robert, K. Provost, & F. Lefaucheux: Crystallization in gels and related
methods; A.C. Dock-Bregeon & D. Moras: Crystallization of nucleic acids and co-crystallization of proteins and nucleic acids; F. Reiss-Husson: Crystallization of membrane proteins; M. Ries-Kaut & A. Ducruix: Phase diagrams; V. Mikol & R. Giegé: The physical chemistry of protein crystallization; E.A. Stura & P. Chen: Soaking of crystals; L.
Sawyer & M.A. Turner: X-ray analysis; K.B. Ward, M.A. Perozzo & W.M. Zuk: Automating crystallization experiments; S. Doublie & C.W. Carter: Preparation of selenomethionyl protein crystals; Appendix; Index.
