Animal Cells as Bioreactors
Series: Cambridge Studies in Biotechnology; 11;
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Product details:
- Publisher Cambridge University Press
- Date of Publication 26 August 1994
- ISBN 9780521412582
- Binding Hardback
- No. of pages196 pages
- Size 236x158x17 mm
- Weight 443 g
- Language English
- Illustrations 21 b/w illus. 39 tables 0
Categories
Short description:
This volume gives an integrated view of how cultured animal cells are used for biopharmaceutical production.
MoreLong description:
Because of their complexity, the new generation of genetically engineered protein drugs can only be made by biotechnological methods, using cultures of animal cells. This book covers all aspects of the technologies needed to turn animal cells into an acceptable and cost-effective tool for drug production. This includes modifying them genetically so that they produce the right product in high yield, getting them to grow reproducibly on an industrial scale, and extracting the required product from them. It also covers biological safety issues, and the verification of the chemical and biological nature of the protein drug produced. The work covers developments in all of these areas and how they all need to be integrated for the design of an effective biotechnological production process. It therefore provides a comprehensive guide to this area of biotechnology.
Review of the hardback: '... provides a useful reference work for all budding biotechnologists.' Wendie Norris, British Society for Development Biology Newsletter
Table of Contents:
1. Introducing the animal cell as a bioreactor; 2. Yield of recombinant product: engineering cells for maximum expression; 3. The generation of biomass, medium design for animal cell culture, fermenter design for animal cell culture, suspension cultures, immobilized cells, process control; 4. Adjusting cellular metabolism for optimum product yield; 5. Downstream processing; 6. Regulatory aspects of using cells as bioreactors, viral contamination of animal cell derived pharmaceuticals; 7. Overview and conclusions; References; Index.
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