MicroRNA Detection and Target Identification

Methods and Protocols
 
Edition number: 2nd ed. 2023
Publisher: Humana
Date of Publication:
Number of Volumes: 1 pieces, Book
 
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Product details:

ISBN13:9781071629819
ISBN10:1071629816
Binding:Hardback
No. of pages:266 pages
Size:254x178 mm
Weight:722 g
Language:English
Illustrations: 5 Illustrations, black & white; 36 Illustrations, color
690
Category:
Short description:

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Long description:
This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Table of Contents:

1. Detection of miRNAs



            Afrah Bawazeer and David C. Prince



 



2. MicroRNA Identification, Target Prediction, and Validation for Crop Improvement



            Vrantika Chaudhary, Sumit Jangra, Apurva Mishra, and Neelam R. Yadav



 



3. MicroRNA Detection with CRISPR/Cas



            Xinyuan Qiu, Chuanyang Liu, Chushu Zhu, and Lingyun Zhu



 



4. Detection of MicroRNAs by Northern Blot



            Claudia Martinho and Sara Lopez
-Gomollon



 



5. MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore



            Sotaro Takiguchi and Ryuji Kawano



 



6. Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor



            Yuwen Zhao and Shue Wang



 



7. Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification



            Yannick Rondelez and Guillaume Gines



 



8. Small RNA Profiling by Next Generation Sequencing Using High
-Definition Adapters



            Rocky Payet and Martina Billmeier



 



9. Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification



            Sarah Ake, Swagatika Kamila, and Gangli Wang



 



10. Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine



            Luděk Záveský and Ondřej Slanař



 



11. Determining miRNA Expression Patterns in Xenopus



            Marco Antonaci, Alice M. Godden, and Grant N. Wheeler



 



12. Overview of Computational and Experimental Methods to Identify Tissue
-Specific MicroRNA Targets



            Ulf Schmitz



 



13. sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non
-Model Species



            Cristina Gómez
-Martín, Ernesto Aparicio
-Puerta, and Michael Hackenberg



 



14. MicroRNA
-Target Identification: A Combinatorial In Silico Approach



            Taufiqul K.M. Arif, Rachel K. Okolicsanyi, Larisa M. Haupt, and Lyn R. Griffiths



 



15. An Efficient CRISPR
-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis



            Alice M. Godden, Marco Antonaci, and Grant N. Wheeler



 



16. Interrogation of Functional miRNA
-Target Interactions by
CRISPR/Cas9 Genome Engineering



            Qianxin Wu, Yale S. Michaels, and Tudor A. Fulga