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  • MicroRNA Detection and Target Identification: Methods and Protocols

    MicroRNA Detection and Target Identification by Dalmay, Tamas;

    Methods and Protocols

    Series: Methods in Molecular Biology; 2630;

      • GET 20% OFF

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      • Publisher's listprice EUR 213.99
      • The price is estimated because at the time of ordering we do not know what conversion rates will apply to HUF / product currency when the book arrives. In case HUF is weaker, the price increases slightly, in case HUF is stronger, the price goes lower slightly.

        90 774 Ft (86 451 Ft + 5% VAT)
      • Discount 20% (cc. 18 155 Ft off)
      • Discounted price 72 619 Ft (69 161 Ft + 5% VAT)

    90 774 Ft

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    Availability

    Estimated delivery time: In stock at the publisher, but not at Prospero's office. Delivery time approx. 3-5 weeks.
    Not in stock at Prospero.

    Why don't you give exact delivery time?

    Delivery time is estimated on our previous experiences. We give estimations only, because we order from outside Hungary, and the delivery time mainly depends on how quickly the publisher supplies the book. Faster or slower deliveries both happen, but we do our best to supply as quickly as possible.

    Product details:

    • Edition number Second Edition 2023
    • Publisher Humana
    • Date of Publication 24 January 2023
    • Number of Volumes 1 pieces, Book

    • ISBN 9781071629819
    • Binding Hardback
    • No. of pages266 pages
    • Size 254x178 mm
    • Weight 722 g
    • Language English
    • Illustrations 5 Illustrations, black & white; 36 Illustrations, color
    • 473

    Categories

    Short description:

    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


    Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.

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    Long description:

    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


    Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.

    More

    Table of Contents:

    Detection of miRNAs.- MicroRNA Identification, Target Prediction, and Validation for Crop Improvement.- MicroRNA Detection with CRISPR/Cas.- Detection of MicroRNAs by Northern Blot.- MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore.- Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor.- Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification.- Small RNA Profiling by Next Generation Sequencing Using High-Definition Adapters.- Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification.- Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine.- Determining miRNA Expression Patterns in Xenopus.- Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets.- sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-Model Species.- MicroRNA-Target Identification: A Combinatorial In Silico Approach.- An Efficient CRISPR-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis.- Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering.

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    MicroRNA Detection and Target Identification: Methods and Protocols

    MicroRNA Detection and Target Identification: Methods and Protocols

    Dalmay, Tamas; (ed.)

    90 774 HUF

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