MicroRNA Detection and Target Identification
EUR 196.19
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| ISBN13: | 9781071629819 |
| ISBN10: | 1071629816 |
| Binding: | Hardback |
| No. of pages: | 266 pages |
| Size: | 254x178 mm |
| Weight: | 722 g |
| Language: | English |
| Illustrations: | 5 Illustrations, black & white; 36 Illustrations, color |
| 690 |
This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
1. Detection of miRNAs
Afrah Bawazeer and David C. Prince
2. MicroRNA Identification, Target Prediction, and Validation for Crop Improvement
Vrantika Chaudhary, Sumit Jangra, Apurva Mishra, and Neelam R. Yadav
3. MicroRNA Detection with CRISPR/Cas
Xinyuan Qiu, Chuanyang Liu, Chushu Zhu, and Lingyun Zhu
4. Detection of MicroRNAs by Northern Blot
Claudia Martinho and Sara Lopez
-Gomollon
5. MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore
Sotaro Takiguchi and Ryuji Kawano
6. Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor
Yuwen Zhao and Shue Wang
7. Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification
Yannick Rondelez and Guillaume Gines
8. Small RNA Profiling by Next Generation Sequencing Using High
-Definition Adapters
Rocky Payet and Martina Billmeier
9. Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification
Sarah Ake, Swagatika Kamila, and Gangli Wang
10. Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine
Luděk Záveský and Ondřej Slanař
11. Determining miRNA Expression Patterns in Xenopus
Marco Antonaci, Alice M. Godden, and Grant N. Wheeler
12. Overview of Computational and Experimental Methods to Identify Tissue
-Specific MicroRNA Targets
Ulf Schmitz
13. sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non
-Model Species
Cristina Gómez
-Martín, Ernesto Aparicio
-Puerta, and Michael Hackenberg
14. MicroRNA
-Target Identification: A Combinatorial In Silico Approach
Taufiqul K.M. Arif, Rachel K. Okolicsanyi, Larisa M. Haupt, and Lyn R. Griffiths
15. An Efficient CRISPR
-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis
Alice M. Godden, Marco Antonaci, and Grant N. Wheeler
16. Interrogation of Functional miRNA
-Target Interactions by CRISPR/Cas9 Genome Engineering
Qianxin Wu, Yale S. Michaels, and Tudor A. Fulga