Biophysical Labeling Methods in Molecular Biology
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Product details:
- Publisher Cambridge University Press
- Date of Publication 26 March 1993
- ISBN 9780521431323
- Binding Hardback
- No. of pages320 pages
- Size 234x156x19 mm
- Weight 630 g
- Language English
- Illustrations 4 b/w illus. 5 tables 0
Categories
Short description:
This 1993 volume covers all aspects of the field of physical labels - the theoretical bases, the experimental techniques - and shows how to interpret the resulting data.
MoreLong description:
Physical labels such as stable nitroxide radicals, luminescent and photochromic chromophores, so-called M&&&246;ssbauer atoms and electron-dense assemblies of heavy atoms have proved to be effective tools in solving many problems at the molecular level in biological systems. These physical labels are used as 'molecular rulers' to measure the distances between chosen groups and to measure the size, form and microrelief of objects. By providing information about these factors, the label provides information that can help the scientist to understand the structure of membranes, nucleic acids, enzymes and proteins and how they function. This 1993 volume covers all aspects of this field: the theoretical bases, the experimental techniques, and it also shows how to interpret the resulting data. It also critically discusses some recent results obtained with these techniques and gives an analysis of likely developments in the future.
"Labeling methods, including fluorescence and spin labeling, are widely used to probe the structure and function of biomolecules....I strongly encourage anyone who wants an intense and well structured introduction into the field of labeling to read Likhenshtein's book. Even the experienced researcher will benefit from it because of the parallel presentation of several labeling methods." Klaus Gawrisch, Analytical Biochemistry
Table of Contents:
1. The methods of spin labeling; 2. Double labeling techniques; 3. Fluorescent labeling methods; 4. Triplet labeling methods; 5. M&&&246;ssbauer, electron-scattering, and other labeling methods; 6. Studies of proteins and enzymes: structure, dynamics and mechanism of action; 7. Structure and dynamics of membrane; 8. Nucleic acids and other biosystems, biological assays; 9. Conclusion; References; Subject index.
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