Type 2 Immunity: Methods and Protocols

Type 2 Immunity

Methods and Protocols
 
Kiadás sorszáma: Softcover reprint of the original 1st ed. 2018
Kiadó: Humana
Megjelenés dátuma:
Kötetek száma: 1 pieces, Previously published in hardcover
 
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A termék adatai:

ISBN13:9781493993185
ISBN10:1493993186
Kötéstípus:Puhakötés
Terjedelem:434 oldal
Méret:254x178 mm
Súly:861 g
Nyelv:angol
Illusztrációk: 27 Illustrations, black & white; 53 Illustrations, color
178
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Rövid leírás:

This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside. 

Hosszú leírás:

This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside. 

Tartalomjegyzék:

1. A Fungal Protease Model To Interrogate Allergic Lung Immunity



J. Morgan Knight, Evan Li, Hui-Ying Tung, Cameron Landers, Jake Wheeler, Farrah Kheradmand, and David B Corry



 



2. Use of the Litomosoides sigmodontis Infection Model of Filariasis to study Type 2 Immunity

Fulton, A., Babayan, S. A., and Taylor, M. D.



 



3. Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool With New Clinical Applications



Min Zhang, Amanda J. Mathew, and William Parker



 



4. A Mouse Model of Peanut Allergy Induced By Sensitization through the Gastrointestinal Tract



Kelly Orgel and Michael Kulis



 



5. Induction and Characterization of the Allergic Eye Disease Mouse Model



Nancy J. Reyes, Rose Mathew, and Daniel R. Saban



 



6. Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung



Hideki Nakano, Keiko Nakano, and Donald N. Cook



 



7. In vitro and In vivo IgE/Antigen Mediated Mast Cell Activation



Hae Woong Choi and Soman N. Abraham



 



8. The use of Human and Mouse Mast Cell and Basophil Cultures to Assess



type-2 Inflammation



Heather L. Caslin, Marcela T. Taruselli, Anuya Paranjape, Kasalina Kiwanuka, Tamara Haque, Alena P. Chumanevich, Carole A. Oskeritzian, and John J. Ryan



 



9. Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation



Jesse Charles Nussbaum and Jorge Felipe Ortiz-Carpena



 



10. Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors



Timothy E. O?Sullivan and Joseph C. Sun



 



11. Characterization of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry



Kathryn D. Tuttle and Laurent Gapin



 



12. Characterization of Mouse gd T Cell Subsets in the Setting of type-2 Immunity



Wanjiang Zeng, Rebecca L. O'Brien, Willi K. Born, and Yafei Huang



 



13. The Identification of Allergen-derived T cell Epitopes



Véronique Schulten and Alessandro Sette



 



14. Generation of Allergen-specific Tetramers for a Murine Model of Airway Inflammation



James J. Moon and Marion Pepper



 



15. The Generation and Use of Allergen-specific TCR Transgenic Animals



Manon Vanheerswynghels, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad, and Bart N. Lambrecht

 



16. Using Cytokine Reporter Mice to Visualize Type-2 immunity in vivo



Mark Dell?Aringa and R. Lee Reinhardt



 



17. Live Imaging of IL-4 expressing T Follicular Helper Cells in Explanted Lymph Nodes



Mark Dell?Aringa, R. Lee Reinhardt, Rachel S. Friedman, and Jordan Jacobelli



 



18. Imaging Precision Cut Lung Slices to Visualize Leukocyte Localization and Trafficking



Miranda R. Lyons-Cohen, Hideki Nakano, Seddon Y. Thomas, and Donald N. Cook



 



19. Study of IgE-producing B cells using the Verigem Fluorescent Reporter Mouse



Zhiyong Yang, James B. Jung, and Christopher D. C. Allen



 



20. Chromatin Preparation from Murine Eosinophils for Genome-wide Analyses



Carine Bouffi, Artem Barski, and Patricia C. Fulkerson



 



21. A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers



Sandy Lisette Rosales, Shu Liang, Isaac Engel, Benjamin Joachim Schmiedel, Mitchell



Kronenberg, Pandurangan Vijayanand, and Grégory Seumois



 



22. An Integrated and Semi-automated Micro-scaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq for Large-scale Epigenetic Studies



Diana Youhanna Jankeel, Justin Cayford, Benjamin Joachim Schmiedel, Pandurangan Vijayanand, and Grégory Seumois



 



23. Library preparation for ATAC-sequencing of Mouse CD4+ T Cells Isolated from the Lung and Lymph Nodes after Helminth Infection



Laura D. Harmacek, Preeyam Patel, Rachel Woolaver, R. Lee Reinhardt, and Brian P. O?Connor



 



24. Identification of Functionally-relevant microRNAs in the Regulation of Allergic Inflammation



Marlys S. Fassett, Heather H. Pua, Laura J. Simpson, David F. Steiner, and K. Mark Ansel



 



25. The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation



Charles B. Smarr and Stephen D. Miller



 



26. Assessing the Mouse Intestinal Microbiota in Settings of type-2 Immune Responses



Mei San Tang, Rowann Bowcutt, and P?ng Loke



27. The use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology



Azzeddine Dakhama and Hong Wei Chu



 



28. A Consistent Method to Identify and Isolate Mononuclear Phagocytes from Human Lung and Lymph Nodes



Sophie L. Gibbings and Claudia V. Jakubzick



 



29. Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation



Bailey Zwarycz, Adam D Gracz, and Scott T Magness



 



30. Utilization of Air-liquid Interface Cultures as an in vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin 13



Jamie L. Everman, Cydney Rios, and Max A. Seibold