Immunometabolism: Methods and Protocols

Immunometabolism

Methods and Protocols
 
Kiadás sorszáma: 1st ed. 2020
Kiadó: Humana
Megjelenés dátuma:
Kötetek száma: 1 pieces, Book
 
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A termék adatai:

ISBN13:9781071608012
ISBN10:10716080111
Kötéstípus:Keménykötés
Terjedelem:284 oldal
Méret:254x178 mm
Súly:765 g
Nyelv:angol
Illusztrációk: 10 Illustrations, black & white; 48 Illustrations, color
247
Témakör:
Rövid leírás:

This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.

Hosszú leírás:
This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.
Tartalomjegyzék:

1. Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq



            Janilyn Arsenio



 



2. Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe



            Felipe Baeza-Lehnert, Carlos A. Flores, Anita Guequen, and L. Felipe Barros



 



3. The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level



            Iivari Kleino, Eliisa Kekäläinen, and Tapio Lönnberg



 



4. Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling



            Kevin J. Williams and Steven J. Bensinger



 



5. Proteomic Network Analysis of Polarized Macrophages



            Jayanta K. Chakrabarty, Abu Hena Mostafa Kamal, A.D.A. Shahinuzzaman, and Saiful M. Chowdhury



 



6. The Purification and Characterization of Exosomes from Macrophages



            Eran Fridman, Lana Ginini, Ziv Gil, and Neta Milman



 



7. The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry



            Inbar Azoulay-Alfaguter and Adam Mor



 



8. Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model



            Tanya N. Augustine



 



9. Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System



            Jennifer M. Monk, Danyelle M. Liddle, Amber L. Hutchinson, and Lindsay E. Robinson



 



10. A Gene Expression Analysis of M1 and M2 Polarized Macrophages



            Nour Eissa, Hayam Hussein, and Jean-Eric Ghia



 



11. Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages



            Casey M. Daniels, Arthur Nuccio, Pauline R. Kaplan, and Aleksandra Nita-Lazar



 



12. The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer



            Bridgette M. Cumming, Vineel P. Reddy, and Adrie J.C. Steyn



 



13. Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification



            Hsi-Ju Wei, John J. Letterio, and Tej K. Pareek



 



14. The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP+) Electrode in Isolated Mitochondria and Intact Cells



            Jo?o S. Teodoro, Ivo F. Machado, Ana C. Castela, Anabela P. Rolo and Carlos M. Palmeira



 



15. The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs)



            Jesse J.R. Masson, Matias Ostrowski, Gabriel Duette, Man kit Sam Lee, Andrew J. Murphy, Suzanne M. Crowe, and Clovis S. Palmer



 



16. The Measurement of Whole Body Glucose Homeostasis in Mice



            Yang Xin Zi Xu, Sudharsana R. Ande, and Suresh Mishra



 



17. Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy



            Andrew D. Sheppard and Joanne Lysaght



 



18. Sex Differences in Immunometabolism: An Unexplored Area



            Suresh Mishra, Geetika Bassi, and Yang Xin Zi Xu



 



19. Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis



            Nnamdi Ikeogu, Chidalu A. Edechi, Gloria Akaluka, Aida Feiz-Barazandeh, and Jude E. Uzonna