Immunity in Insects

Immunity in Insects

 
Kiadás sorszáma: 1st ed. 2020
Kiadó: Humana
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Kötetek száma: 1 pieces, Book
 
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A termék adatai:

ISBN13:9781071602584
ISBN10:1071602586
Kötéstípus:Keménykötés
Terjedelem:246 oldal
Méret:254x178 mm
Súly:723 g
Nyelv:angol
Illusztrációk: 7 Illustrations, black & white; 43 Illustrations, color; 43 Tables, color
191
Témakör:
Rövid leírás:

This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



 



Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field.Incl 

Hosszú leírás:

This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



 



Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field.



?With its broad and lucid treatment of insect immunity, this volume paves the way for new recruits and encourages current researchers to expand and refine their approaches.? (James B. Nardi, The Quarterly Review of Biology, Vol. 95 (4), December, 2020)
Tartalomjegyzék:

Part I: Searching for Genes and Proteins Involved in the Immune Response



1. Characterization of Insect Immune Systems from Genomic Data



Robert M. Waterhouse, Brian P. Lazzaro, and Timothy B. Sackton



 



2. Analyzing Immunity in Non-model Insects using de novo Transcriptomics



Shulin He, Paul R. Johnston, and Dino P. McMahon



 



3. Preparation of Insect Protein Samples for Label-free Proteomic Quantification by LC-mass Spectrometry



Alexandro Rodríguez-Rojas and Jens Rolff



 



Part II: Cellular Response



4. Quantification of Blood Cells in Drosophila melanogaster and other Insects



Sean Corcoran and Katja Brückner



 



5. Methods to Quantify in vivo Phagocytic Uptake and Opsonization of Live or Killed microbes in Drosophila melanogaster



Samuel Liégeois, Wenhui Wang, and Dominique Ferrandon



 



6. Analysis of Cellular Immune Responses in Lepidopteran Larvae



Andrea Becchimanzi, Ilaria Di Lelio, Francesco Pennacchio, and Silvia Caccia



 



Part III: Humoral Response: Melanization, Lysozyme, and Antimicrobial Peptides



7. Detection of Enzyme Distribution, Expression, Activation, and Activity of Insect Prophenoloxidase



Kai Wu, Bing Yang, Jing Wang, Yufa Luo, Yuyang Ni, and Wuren Huang



 



8. Basic Methods to Evaluate Humoral Immunity Processes in Lepidoptera larvae



Maristella Mastore and Maurizio Francesco Brivio



 



Part IV: Humoral Response: Antimicrobial Peptides as Alternatives to Conventional Antibiotics



9. Methods for the in vitro Examination of the Antibacterial and Cytotoxic Activities of Antimicrobial Peptides



Bruno Casciaro, Floriana Cappiello, Maria Rosa Loffredo, and Maria Luisa Mangoni



 



10. Characterization of Antimicrobial Peptide - Membrane Interaction Using All-atom Molecular Dynamic Simulation



Shruti Mukherjee, Rajiv K. Kar, Anirban Bhunia



 



Part V: Insect Immunity and Environmental Interactions



11. Manipulating the Mosquito Microbiota to Study its Function



Ottavia Romoli and Mathilde Gendrin



  12. Discovery and Analysis of RNA Viruses in Insects

Lumi Viljakainen and Jaana Jurvansuu



 



VI: Infection Models



13. A Workflow for Infection of Drosophila with Entomopathogenic Nematodes to Monitor Immune Gene Transcriptional Activity



Christa Heryanto, Eric Kenney, and Ioannis Eleftherianos



  14. Oral Infection in a Germ-Free Bombyx mori Model

Daniel Brady, Alessio Saviane, Ottavia Romoli, Gianluca Tettamanti, Federica Sandrelli, and Silvia Cappellozza



 



15. Silkworm Infection Model for Evaluating Pathogen Virulence



Yasuhiko Matsumoto and Kazuhisa Sekimizu