Toxoplasma gondii
Methods and Protocols
Series:
Methods in Molecular Biology;
2071;
Edition number: 1st ed. 2020
Publisher: Humana
Date of Publication: 23 November 2019
Number of Volumes: 1 pieces, Book
Normal price:
Publisher's listprice:
EUR 160.49
EUR 160.49
Your price:
60 928 (58 026 HUF + 5% VAT )
discount is: 8% (approx 5 298 HUF off)
The discount is only available for 'Alert of Favourite Topics' newsletter recipients.
Click here to subscribe.
Click here to subscribe.
Availability:
Estimated delivery time: Currently 3-5 weeks.
Not in stock at Prospero.
Can't you provide more accurate information?
Not in stock at Prospero.
Product details:
| ISBN13: | 9781493998562 |
| ISBN10: | 14939985611 |
| Binding: | Hardback |
| No. of pages: | 470 pages |
| Size: | 254x178 mm |
| Weight: | 1120 g |
| Language: | English |
| Illustrations: | 14 Illustrations, black & white; 63 Illustrations, color; 52 Tables, color |
| 164 |
Category:
Short description:
This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.
Long description:
This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.
Table of Contents:
13. Experimental Approaches for Examining Apicoplast Biology
Preface?
Table of Contents?
Contributing Authors?
1. What a Difference 30 years Makes! A Perspective on Changes in research Methodologies used to Study Toxoplasma gondii
John C. Boothroyd
2. ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms
Omar S. Harb and David S. Roos
3. Isolation and Genotyping of Toxoplasms gondii Strains
Chunlei Su and Jitender P. Dubey
4. Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of their Sporozoites for Downstream Manipulation
Sarah L. Sokol, Sheen Wong, Jon P. Boyle, and Jitender P. Dubey
5. Assay for Monitoring Toxoplasms gondii Infectivity in the Laboratory Mouse
Qiuling Wang and L. David Sibley
6. PCR Screening of Toxoplasms gondii Single Clones Directly from 96-Well Plantes without DNA Purification
Federica Piro, Vern B. Carruthers, and Manlio Di Cristina7. CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma gondii
Damien Jacot and Dominique Soldati-Favre
8. Assessing Rhyptry Secretion in T. gondii
Catherine Suarez, Melissa B. Lodoen, and Maryse Lebrun
9. High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii
Kevin M. Brown, L. David Sibley, and Sebastian Lourido
10. Plate-Based Quantification of Stimulated Toxoplasma egress
Emily Shortt and Sebastian Lourido
11. Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii
Stephen A. Vella, Abigail Calixto, Beejan Asady, Zhu-Hong Li, and Silvia N.J. Moreno
12. Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging
Georgios Pavlou and Isabelle Tardieux
Marco Biddau, Jana Ovciarikova, and Lilach Sheiner
14. Measuring Solute Transport in Toxoplasma gondii Parasites
Esther Rajendran, Kiaran Kirk, and Giel G. van Dooren
15. Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo
Joshua Mayoral, Manlio Di Cristina, Vern B. Carruthers, and Louis M. Weiss
16. Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In Situ
Carla M. Cabral, Emily F. Merritt, and Anita A. Koshy
17. Model Systems for Studying Mechanisms of Ocular Toxoplasmosis
Justine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, and Jo?o M. Furtado18. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii
Peter J. Bradley, Shima Rayatpisheh, James A. Wohlschlegel, and Santhosh M. Nadipuram
19. Assays to Evaluate Toxoplasma-Macrophage Interactions
Debanjan Mukhopadhyay and Jeroen P.J. Saeij20. Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells
Catalina Alvarez, Ana Claudia Campos, Jonathan C. Howard, Joana Loureiro, Urs Benedikt Müller, and Ana Lina Rodrigues
21. Image-based Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host Response to Microbe Analysis Pipeline
Daniel Fisch, Artur Yakimovich, Barbara Clough, Jason Mercer, and Eva-Maria Frickel
22. Metabolomic Analysis of Toxoplasma gondii Tachyzoites
Elizabeth King, Simon Cobbold, Alessandro D. Uboldi, Christopher J. Tonkin, and Malcolm J. McConville
23. Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phospho- and Total Proteome in Toxoplasma gondii
Malgorzata Broncel and Moritz Treeck
