The Epithelial-to Mesenchymal Transition
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| ISBN13: | 9781071607787 |
| ISBN10: | 1071607782 |
| Binding: | Hardback |
| No. of pages: | 429 pages |
| Size: | 254x178 mm |
| Weight: | 1034 g |
| Language: | English |
| Illustrations: | 6 Illustrations, black & white; 79 Illustrations, color |
| 246 |
This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.
This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.
Part I: Perspectives on the Past and Future of EMT Research
1. A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition
Erica J. Hutchins and Marianne E. Bronner
2. Perspective on Epithelial Mesenchymal Transitions in Embryos
David R. McClay
3. EMT, One of Many Morphological Transitions in Cellular Phase Space
Denise J. Montell
4. Are you Interested or Afraid of Working on EMT?
M. Angela Nieto
5. Partial EMT/MET: An Army of One
Sofiane Hamidi, Hiroki Nagai, and Guojun Sheng
6. EMT; An Update
Jean Paul Thiery
Part II: Review on Mesenchymal-to-Epithelial Transitions
7. Mesenchymal to Epithelial Transitions in Development and Cancer
John-Poul Ng-Blichfeldt and Katja Röper
Part III: Methods to Study EMT in In Vivo Systems
8. Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos
Lingkun Gu and Mo Weng
9. Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration
Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Härtel, and Claudia Linker
10. Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo
Mary Cathleen McKinney and Paul M. Kulesa
11. Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors
Sofia Golenkina, Rosemary Manhire-Heath, and Michael J. Murray
12. Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging
Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, and Isabelle Migeotte
13. Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo
Joshua Greig and Natalia A. Bulgakova
14. Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster
Jamie Adams, Andreu Casali, and Kyra Campbell
Part IV: In Vivo Imaging of MET
15. 4D Live-Imaging and Analysis of Chick Embryo Somites
Gi Fay Mok, James McColl, and Andrea Münsterberg
16. In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation
Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Bénazéraf, and Elisa Martí
17. Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos
Gordana Scepanovic, Alexandru Florea, and Rodrigo Fernandez-Gonzalez
Part V: Ex Vivo/InVitro Methods to Study EMT/MEt
18. Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering
Minerva Bosch-Fortea and Fernando Martín-Belmonte
19. CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay
Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat, Anna Labernadie
20. Using Xenopus Neural Crest Explants to Study
Epithelial-mesenchymal Transition
Nad?ge Gouignard, Christian Rouvi?re, and Eric Theveneau
21. Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition
Hye Young Kim and Lance A. Davidson
22. Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition
Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P. Girard, and Nicolas Borghi
Part VI: Methods to Study Epithelial- Mesenhymal Plasticity at the Single Cell, Subcellular, and Molecular Level
23. Methodologies for Following EMT in vivo at Single Cell Resolution
Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio, Lingyu Wang, Gregory A. Wray, and David R. McClay
24. Isolation and Identification of EMT Subtypes
Robert J. Norgard and Ben Z. Stanger
25. Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer
Sugandha Bhatia, Erik W Thompson, and Jennifer H Gunter
26. Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells
Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, and Hervé Acloque
27. Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling
Noémie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube, Kerstin Bystricky, and Anne-Claire Lavigne
28. Mathematical Modeling of Plasticity and Heterogeneity in EMT
Shubham Tripathi, Jianhua Xing, Herbert Levine, and Mohit Kumar Jolly
29. Control of Cell Migration Using Optogenetics
Leo Valon and Simon de Beco
