Cell Reprogramming for Immunotherapy: Methods and Protocols

Cell Reprogramming for Immunotherapy

Methods and Protocols
 
Edition number: 1st ed. 2020
Publisher: Humana
Date of Publication:
Number of Volumes: 1 pieces, Book
 
Normal price:

Publisher's listprice:
EUR 239.79
Estimated price in HUF:
98 949 HUF (94 237 HUF + 5% VAT)
Why estimated?
 
Your price:

91 033 (86 698 HUF + 5% VAT )
discount is: 8% (approx 7 916 HUF off)
The discount is only available for 'Alert of Favourite Topics' newsletter recipients.
Click here to subscribe.
 
Availability:

Estimated delivery time: Currently 3-5 weeks.
Not in stock at Prospero.
Can't you provide more accurate information?
 
  Piece(s)

 
 
 
 
Product details:

ISBN13:9781071602027
ISBN10:10716020211
Binding:Hardback
No. of pages:343 pages
Size:254x178 mm
Weight:893 g
Language:English
Illustrations: 7 Illustrations, black & white; 34 Illustrations, color; 38 Tables, color
174
Category:
Short description:

This volume details key protocols for developing strategies in immunotherapy.  Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



 Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Long description:

This volume details key protocols for developing strategies in immunotherapy.  Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



 Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of Contents:

Part I: Reprogramming T and NK Cells



1. T Cell Reprogramming against Cancer



Samuel G. Katz and Peter M. Rabinovich



 



2. Identification of Cell Surface Targets for CAR T Cell Immunotherapy



Diana C. DeLucia and John K. Lee



 



3. Microfluidic Approach for Highly Efficient Viral Transduction



Reginald Tran and Wilbur A. Lam



 



4. Single-cell Cytokine Analysis to Characterize CAR-T Cell Activation



Amanda Finck and Rong Fan



 



5. Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells



Roddy S. O?Connor and Michael C. Milone



 



6. Engineering of Natural Killer Cells for Clinical Application



Noriko Shimasaki and Dario Campana



 



7. Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells



Arash Nanbakhsh and Subramaniam Malarkannan



 



8. In vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection



Nathan J Schloemer, Alex M. Abel, Monica S Thakar, and Subramaniam Malarkannan



 



9. Immunomodulation of NK Cell Activity



Carolina I. Domaica, Jessica M. Sierra, Norberto W. Zwirner, and Mercedes B. Fuertes



 



Part II: Reprogramming Diverse Immunocytes



10. An Overview of Advances in Cell-based Cancer Immunotherapies Based on the Multiple Immune-cancer Cell Interactions



Jialing Zhang, Stephan S. Späth, Sherman M. Weissman, and Samuel G. Katz



 



11. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy



Douglas Hanlon, Olga Sobolev, Alessandra Ventura, Aaron Vassall, Nour Kibbi, Patrick Han, Alp Yurter, Eve Robinson, Renata Filler, Kazuki Tatsuno, Richard L. Edelson



 



12. Reprogramming Exosomes for Immunotherapy



Qinqin Cheng, Xiaojing Shi, and Yong Zhang



13. Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment



Ketki Bhise, Samaresh Sau, Rami Alzhrani, and Arun K. Iyer



14. CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow Derived Macrophages



Will Bailis



 



15. Genome-Wide CRISPRi/a Screening in an In Vitro Co-culture Assay of
Human Immune Cell with Tumor Cells



Jialing Zhang, Stephan S. Späth, and Samuel G. Katz



 



16. The Propagation and Quantification of Two Emerging Oncolytic Viruses:
 Vesicular Stomatitis (VSV) and Zika (ZIKV)



Robert E. Means, Sounak Ghosh Roy, and Samuel G. Katz



 



Part III: Monitoring Reprogrammed Immune Cells In Vivo



17. Radiolabeling and Imaging of Adoptively Transferred Immune Cells by Positron Emission Tomography



Amer M. Najjar



 



18. Functional Analysis of Human Hematopoietic Stem Cells in vivo in Humanized Mice



Yuanbin Song, Rana Gbyli, Xiaoying Fu, and Stephanie Halene



 



Part IV: Clinical Design for Cell Therapy



19. Monitoring Allogeneic CAR-T Cells using Flow Cytometry



Agnieszka Jozwik, Alan Dunlop, Katy Sanchez, and Reuben Benjamin



 



20. Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma



Bryden Considine and Michael E Hurwitz



 



21. Place of Academic GMP Facilities in Modern Cell Therapy



Alexey Bersenev and Andrew Fesnak