Basic Methods in Antibody Production and Characterization
Series:
Routledge Revivals;
Edition number: 1
Publisher: CRC Press
Date of Publication: 31 March 2021
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Product details:
| ISBN13: | 9780367202446 |
| ISBN10: | 0367202441 |
| Binding: | Paperback |
| No. of pages: | 284 pages |
| Size: | 234x156 mm |
| Weight: | 408 g |
| Language: | English |
| Illustrations: | 8 Tables, black & white |
| 0 |
Category:
Short description:
First published in 2001: The book focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications. Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications.
Long description:
First published in 2001: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies.
Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.
Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.
Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.
Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.
Table of Contents:
1. Starting Out. 2. Animal Welfare and Regulatory Issues. 3. Antigens. 4. Adjuvants. 5. Polyclonal Antibodies. 6. Monoclonal Antibody Production. 7. Screening Techniques. 8. Antibody Production by Ascites Growth. 9. Growth of Cell Lines in Bioreactors. 10. Phage Display. 11. Antibody Purification Methods. 12. Molecular Characterization of Immunoglobin Genes. 13. Antibody Storage. 14. Chemically Modifying Antibodies. 15. Western Blotting. 16. Immunohistochemical Methods.
