Antibody Engineering
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| ISBN13: | 9781493993536 |
| ISBN10: | 1493993534 |
| Binding: | Paperback |
| No. of pages: | 543 pages |
| Size: | 254x178 mm |
| Weight: | 1053 g |
| Language: | English |
| Illustrations: | 33 Illustrations, black & white; 73 Illustrations, color; 68 Tables, color |
| 169 |
This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.
Part I: In Silico Methods
1. Antibody Design and Humanization via In Silico Modeling
Vinodh B. Kurella and Reddy Gali
2. Antibody Affinity Maturation by Computational Design
Daisuke Kuroda and Kouhei Tsumoto
3. Use of IMGT? Databases and Tools for Antibody Engineering and Humanization
Marie-Paule Lefranc, François Ehrenmann
, Sofia Kossida, Véronique Giudicelli, and Patrice Duroux
Part II: Generation of Diversity
4. Construction of Human Na?ve Antibody Gene Libraries
Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, and Katia Scotlandi
5. Construction of Synthetic Antibody Libraries
Déborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau
6. Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments
Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka, and Harald Kolmar
7. Display Technologies for Generation of Ig Single Variable Domains
Vladimir Bobkov, Bas van der Woning, and Hans de Haard
8. A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries
Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir, Lars Toleikis, Harald Kolmar, Stefan Becker, and Stefan Zielonka
Part III: Selections of Lead Candidate
9. Phage Display and Selections on Purified Antigens
Magali Colazet and Patrick Chames
10. Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display
Martina L. Jones, Stephen M. Mahler, and Sumukh Kumble
11. Selection of Antibody Fragments against Structured DNA by Phage Display
Mahdi Zeraati, Marcel E. Dinger, and Daniel Christ
12. Selection of Antibody Fragments by Yeast Display
Nathalie Scholler
13. Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display
Birgit Dreier and Andreas Plückthun
14. In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display
Naoto Nemoto, Shigefumi Kumachi, and Hidenao Arai
15. Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood
Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ, and Joanne H. Reed
Part IV: Production of Recombinant Antibodies
16. Expression of IgG Monoclonals with Engineered Immune Effector Functions
Rodrigo Vazquez-Lombardi, Damien Nevoltris, Romain Rouet, and Daniel Christ
17. An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines
Jessna H.M. Yeo, Mariati, and Yuansheng Yang
18. Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins
Martin Siegemund, Nadine Beha, and Dafne Müller
Part V: Fc Engineering
19. Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial ?-Fucosidases
Chao Li, Tiezheng Li, and Lai-Xi Wang
20. Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity
Christian Kellner, Stefanie Derer, Katja Klausz, Sophia Rosskopf, Tim Wirt, Thies Rösner, Anna Otte, Elisa Cappuzzello, and Matthias Peipp
21. Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0
Abhishek Saxena, Bingxin Bai, Shin-Chen Hou, Lianlian Jiang, Tianlei Ying, Shane Miersch, Sachdev S. Sidhu, and Donghui Wu
Part VI: Characterization, Optimization, and Innovative Formats
22. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
Stephen Hearty, Paul Leonard, Hui Ma, and Richard O?Kennedy
23. Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development
Edward Franklin, Orla Cunningham, and Brian Fennell
24. The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies
Peter M. Bowers, William J. Boyle, and Robert Damoiseaux
25. Selection and Use of Intracellular Antibodies
Sandrine Moutel, Clément Nizak, and Franck Perez
26. Site-Specific Radioactive Labeling of Nanobodies
Maxine Crauwels, Sam Massa, Charlotte Martin, Cecilia Betti, Steven Ballet, Nick Devoogdt, Catarina Xavier, and Serge Muyldermans
